Optical Screening Analysis Suite
JSB Lab, University of Bonn
jsb@uni-bonn.de

1. Analyze NIS-Seq raw imaging data

2. Mapping of phenotype to insitu images: one-step (same objective and tile pattern), step 1 (coarse), step 2 (refine)

3. Link nuclei between phenotype and in-situ images

4. Quantify correlation phenotype, Quantify specking phenotype

5. Combine phenotype and NIS-Seq data

6. Visual Data Exploration

7. Obtain Collages


JSB lab open source microscope control software

GitHub repository containing image analysis source code and scripts used in Fig. 1E, 1F, 2A, 2D, 3A, 3E

Download step-by-step imaging protocol
Download step-by-step data analysis protocol
Download robot programs (For Beckman Coulter BIOMEK i7 with span-8 and heating station)

Download example data:
Brunello human sgRNA library and scrambled control
Fig. 1E NIS-Seq HeLa
Fig. 1E NIS-Seq MaMel 65

This software uses the following open source libraries:
pako.js
utif.js
fourier.js