NIS-Seq Analysis Suite v1.0 - Image Mapping (coarse, step 1/2)
JSB lab 2020-2024

In-situ images (TIFF, 2048x2048, 16 bit, sorted by tile > channel, only load one cycle):
channels

Stage positions (time-well-tile-x-y in µm, with header)
Shrink masks

Phenotype images (TIFF, 2048x2048, 16 bit, sorted by tile > channel):
channels

Stage positions (time-well-tile-x-y in µm, with header)
Shrink masks

Internal search tile size (power of 2):
Scaling factor (in-situ pixel size / phenotype pixel size):
Rotation (deg):
Cap image signal at:

1. Generate dictionary 2. Start alignment

Inspect raw images:



In-situ / phenotype
Tile
Channel
Brightness

test