NIS-Seq Analysis Suite v1.0 - Image Mapping (fine, step 2/2)
JSB lab 2020-2024

In-situ images (TIFF, 2048x2048, 16 bit, sorted by tile > channel, only load one cycle):
channels

shrink masks

Phenotype images (TIFF, 2048x2048, 16 bit, sorted by tile > channel):
channels

shrink masks

Coarse alignment results:
Alignment file (tab delimited, tile A - tile B, no header)

Scaling factor (in-situ pixel size / phenotype pixel size):
Cap image signal at:

Start alignment

Inspect raw images:



In-situ / phenotype
Tile
Channel
Brightness

test