NIS-Seq Analysis Suite v1.0 - Obtain collages of lists of selected cells
JSB lab 2020-2024

Phenotype cell masks (TIFF, 1 channel, 2048x2048, 16 bit, sorted by tile):


Phenotype images (TIFF, 2048x2048, 16 bit, sorted by channel > tile):

Channels:

List of cells to be included in collages:
(tab delimited, tile - cell - x - y - sequence/gene)

Limit number of collages:
Tile size for each cell: px
Collage rows size: (e.g. 5 means obtaining 5x5 grids)
Collage color channel: (counting from 1)
Enlarge masks by 2 pixels:
Scale down 2-fold:
Overwrite tiles:
Aggregate collages in memory Stop

Minimum cells per collage:
Maximum cells per collage:
Limit files to be saved:
Save Collages

Inspect raw images:



Type (masks / raw images)
Tile
Channel
Brightness

test