jsMicroscope v1.0 (Chrome only)
Developed 2022-2024 by the JSB Lab, University of Bonn (jsb-lab.bio)
Please note: This script directly interacts with the USB interface of the Nikon Ti2 microscope. The use of this script is not specified by Nikon, and Nikon indicates this may result in accidents that damage the product, people, or property at the user's own risk. Nikon's USB commands may be subject to change without notice.
1. Startup: - Leave on: Celesta Light Engine, W1 control box, Hamamatsu Flash camera, this software
- Ensure the local camera server is running in the background (download)
- Turn on: Nikon Ti2 control box, CSU-W1 (key)
- If not connected, connect:
2. Move to well
3. Focus
PFS position (optionally comma separated): AU, measured:
um
4. Take a test image
Exposure: ms (multiple of 30)
5. (optional) Load a reference image from the same position and channel to fine-align the stage:
6. Image multiple positions and channels: Folder:
Plate name:
List of wells: (B2,B3 comma separated, no spaces, empty = current position, A1-B2 for range of wells)
Maximum rows / columns per well: (for example, 4 means that 4 x 4 images are taken at maximum, if they fit into the well)
Spacing of columns (um): um (automatic)
Edge margin: (0-2, higher values make the well smaller)
Timepoints: every seconds
Bursts: (short local videos at max speed)
Z-Stacks: every micrometers, upwards from PFS position
Wait for PFS to stabilize at every position
Imaging: